In vitro antimicrobial activity of citrus aurantifolia and its phytochemical screening. Antibacterial activity of citrus aurantifolia leaves extracts against some enteric bacteria of public health importance volume 1 issue 2 abubakar u zage 1, sani tajo t 2 and muhammad ali 3 1. Evaluation of clones of acid lime citrus aurantifolia for resistance to citrus tristeza virus. Pdf on dec 10, 2018, muhammad ali and others published antibacterial activity of citrus aurantifolia leaves extracts against some enteric bacteria of public health importance find, read and. To evaluate the antimicrobial efficacy of citrus aurantifolia linn ca against some microorganisms bacteria and fungus were staphylococcus aureus, escherichia coli, klebsiella. Antimicrobial effects of citrus sinensis peel extracts against dental caries bacteria. On the other hand, the yield of essential oil from the citrus aurantifolia peel was 0. Key lime citrus aurantifolia inhibits the growth of triple. Over the last decades, many other virtues and medicinal benefits of citrus fruits have been discovered besides the antiscurvy property rapisarada et al. Water is the most basic asset required for survival. Its oil is mainly used as antidepressant because it promoted. Antimicrobial activity and chemical composition of citrus aurantifolia christm. If the inline pdf is not rendering correctly, you can download the pdf file. It is valued for its nutritional qualities and numerous health benefits.
Antimicrobial activity of the peel extract of lemon is directly concerned with the components that they contain. The antimicrobial effects of aqueous extracts of peel and juice from fresh and dried citrus and sweet lemon against 6 grampositive and 8 gramnegative bacterial and one yeast isolates, including staphylococcus aureus, staphylococcus. Ethnomedical importance of citrus aurantifolia christm swingle. Aqueous and ethanol cold and hot extracts prepared from peel of citrus sinensis were screened for in vitro antimicrobial activity against streptococcus mutans and lactobacillus acidophilus, using agar well diffusion method. Characterization of citrus aurantifolia swingle fam. Oct 22, 2008 the aim of this work was to determine the effectiveness of the essential oils eos of lemon citrus lemon l. Fruit juices of citrus tangerine tangerine, citrus paradisi grape, citrus limon lemon, and citrus aurantifolia lime were evaluated. Shetty 1, prabu mahinsyedismail 2, shaji varghese 3, bibin thomasgeorge 4, pathinettam kandathil. Nov, 2006 we investigated the potency of citrus aurantifolia lime fruit, against pathogens, in the different forms in which this fruit plant is used locally juice of the fruit, burnt rind of the fruit commonly known as epaijebu in the yoruba dialect and the oil obtained from steam distillation of the fruit. Preliminary phytochemical and antibacterial screening of. Antimicrobial effects of citrus sinensis peel extracts. Antibacterial activity of citrus limonon acnevulgaris pimples.
Studies of antimicrobial activity on peel extracts from different citrus species. Antimicrobial screening antimicrobial assay of fruit and leaf waste extracts a, b, c were done against bacteria and the technique employed was agar well diffusion method at varying volumes. Setzer 1,2, id 1 aromatic plant research center, 230 n 1200 e, suite 102, lehi, ut 84043, usa. Antibacterial activity of lime citrus aurantifolia. Extracts of citrus limon were evaluated for their antioxidant and antimicrobial the highest antibacterial activity was obtained against enterococcus faecalis and bacillus subtilis most susceptible bacteria were salmonella typhimurium, shigella sonnei, e. Antimicrobial agents may have side effect such as microbials resistances to antimicrobials. Evaluation of the antimicrobial properties of different parts of citrus. Antibacterial activity of lime citrus aurantifolia essential. Pdf antibacterial activity of citrus aurantifolia leaves. The antibacterial activity of lime citrus aurantifolia essential oil leo and limonene was tested against seven gramnegative and nine grampositive fish pathogenic bacteria isolated from cultured olive flounder. Grapefruit, citrus limon lemon, citrus reticulata tangerine, citrus grandis shaddock, citrus aurantium sour orange, citrus medica citron, and citrus aurantifolia lime are some important.
The natural agents for antimicrobial activity have been detected in citrus aurantifolia fruit peel and leaves. Antimicrobial activities of essential oils and crude extracts. Antimicrobial activity for crude watery extract of seeds of citrus aurantifolia lime fruit against. Many of them are with remarkable antimicrobial activities. In west africa, lime is an essential ingredient in herbal medicine to treat. Citrus aurantifolia is an important medicinal and food plant widely cultivated in many parts of the world. Osbeck and bitter orange citrus aurantium l, it was found that most of the compounds in these citric essential oils are terpenes, such as. Ppt studies of antimicrobial activity on peel extracts from. Citrus essential oils ceos are a mixture of volatile compounds consisting mainly of monoterpene hydrocarbons and are widely used in the food and pharmaceutical industries because of their. In vitro assessment of antibacterial activity of citrus. Citrus aurantifolia is a perennial tree, with evergreen leaves, thorny stem, whitish flowers, globase fruits with many seeds, green when unripe and greenishyellow when ripe, with sour taste ikeyi and omeh, 2014.
A is citrus aurantifolia fruit peel extract, b is citrus aurantifolia leaf extract, c is citrus limetta fruit peel extract. Antifungal activity of citrus essential oils journal of. The antibacterial activities of the various concentrations of the essential oil against bacterial isolates that were earlier subjected to the antibacterial effect of some conventional antibiotics are shown in figure 1. In vitro antimicrobial activity of citrus aurantifolia and its. Evaluation of the phytochemical and antimicrobial potential. Regulation of flushing and flowering in acid lime citrus aurantifolia swing. In addition the fiber of citrus fruit also contains bioactive compounds such as polyphenols, the most important being vitamin c,and they certainly prevent and cure vitamin c deficiencythe cause of scurvy 6. Pdf phytochemicals from citrus limon juice as potential. Pdf the antimicrobial activities of bitter leaf vernonia amygdalina extract and lime citrus aurantifolia juice were compared in this study. Citrus aurantifolia was also used for cold fevers, sore throats, sinusitis and bronchitis, as well as helping asthma.
Citrus aurantifolia, the family rutaceae, which consists of 150 genera and 900 species, is gaining grounds as important source for treatment in complementary medicine, and commonly called limone. In vitro antimicrobial activity of citrus aurantifolia and. We investigated the potency of citrus aurantifolia lime fruit, against pathogens, in the different forms in which this fruit plant is used locally juice of the fruit, burnt rind of the fruit commonly known as epaijebu in the yoruba dialect and the oil obtained from steam distillation of the fruit. Presently, most of the water resources are contaminated with pathogens. Phytochemical, antimicrobial, and antioxidant activities of. Citrus aurantifolia leaves and fruit peel 1287 bacterial strains and antimicrobial assays in vitro antimicrobial activities of cleo and cpeo were determined by minimum inhibitory concentration mic assays which were based on the broth microdilution method clsi 2009. Citrus aurantifolia juice has been useful for the treatment of various infections and green synthesis of silver nanoparticle using lime juice may offer added advantages. Study of phytochemical screening and antimicrobial. The hot and cold aqueous and ethanol extracts of the citrus sinensis were used for the antimicrobial screening using the agar well diffusion method. Antimicrobial activities against five bacterial and three fungal strains were evaluated. Thin layer chromatography tlc of hexane and chloroform extracts showed three spots with different concentrations. Pdf we investigated the potency of citrus aurantifolia lime fruit, against pathogens, in the different forms in which this fruit plant is used. The genus citrus includes several important species worldwide, oranges by 56%.
Antimicrobial activity and chemical composition of citrus. Antimicrobial activity for crude watery extract of seeds of citrus. The media was punched with 7mm diameter wells and were filled with various concentrations of the extracts 5mgml, 10mgml, 15mgml, 20mgml and 25mgml. The antimicrobial effects of aqueous extracts of peel and juice from fresh and dried citrus and sweet. Phytochemical screening, antioxidant, antibacterial. The genus citrus includes several important species worldwide. Chemical composition and antibacterial activity of essential. Setzer 1,2, id 1 aromatic plant research center, 230. The use of herbal therapy has been considered as alternative of antimicrobial therapy. The lowest concentration of every extract considered as the minimal inhibitory concentration mic values were determined for both test organisms. The aim of this work was to determine the effectiveness of the essential oils eos of lemon citrus lemon l. In vitro study of antibacterial activity by citrus aurantifolia fruit peel. Introduction according to the world health organization, a medicinal plant is any plant in which one or more of its organs contain substances that can be used for the synthesis of useful drugs who, 1977. Citrus fruits are also known to contain bioactive compounds such as phenolics, flavonoids, vitamins, and essential oils which are believed to be responsible for a range of protective health.
In this study, antibacterial as well as antifungal activities of essential oils and ethyl acetate extracts from various tropical citrus cultivars available in. Antibacterial activity of lime citrus aurantifolia essential oil and. Rapid water disinfection using zno nanoparticles synthesized. The interest in citrus fruits has been increased for its different important roles like antioxidant, nutritive, antimicrobial, antioxidant and many more other properties.
The chemical composition of essential oils hydrodistilled from the leaves of citrus aurantifolia swingle and citrus reticulata blanco from nigeria were being reported. The antibacterial activities of the various concentrations of the essential oil against bacterial isolates that. Antimicrobial and antioxidant efficacy of citrus limon l. The effectiveness antimicrobial activity test of citrus peel. Citrus are well known as one of the worlds major fruit crops that. Studies on the antimicrobial effects of garlic allium.
The key lime is usually picked while it is still green, but it becomes yellow when ripe. Antibacterial activity of essential oil from citrus. Citrus aurantifolia belongs to the family rutaceae orange family bakare et al. The study was conducted to determine the phytochemical composition and antibacterial activity of citrus aurantifolia leaves extracts against.
Pdf evaluation of the antimicrobial properties of different parts of. Citrus aurantifolia is a perennial tree, with evergreen leaves, thorny stem, whitish flowers, globase fruits with many. Lime essential oil from citrus aurantifolia peel has antimicrobial activity against bacteria and fungi, 17, 18. Shetty 1, prabu mahinsyedismail 2, shaji varghese 3, bibin thomasgeorge 4. Pdf antimicrobial effect of citrus aurantifolia juice and veronica. Citrus is the most extensively produced tree fruit crop in the world.
In the philippines, the use of herbal medicines as remedy for diseases is still common. The antimicrobial activity of epaijebu in different solvents was also compared. Pdf evaluation of the antimicrobial properties of different. To evaluate the antimicrobial efficacy of citrus aurantifolia linn ca against some microorganisms bacteria and fungus were staphylococcus aureus, escherichia coli, klebsiella pneumonia, pseudomonas spp, aspergillus niger, aspergillus fumigates, mucor spp and pencillium. In fact, lemon and lime production reached nearly 27. Antimicrobial activity of different aqueous lemon extracts. Antibacterial activity of citrus aurantifolia leaves extracts against some enteric bacteria of public health importance volume 1 issue 2 abubakar u zage 1, sani tajo t 2 and muhammad ali 3 1 department of microbiology, kano state university of science and technology wudil kano, nigeria. Study of phytochemical screening and antimicrobial activity. Phytochemical composition and comparative evaluation of. Citrus aurantifolia citrus aurantifolia was used traditionally as laxative, appetizer, stomachic, digestive, anthelmintic, dyspepsia, flatulence and helmenthiasis55.
In vitro study of antibacterial activity by citrus. Key lime citrus aurantifolia inhibits the growth of triple drug resistant helicobacter pylori s. As the use of safe water which is clean and pathogenfree is the. Streptococcus salivarius atcc 25975, streptococcus sobrinus atcc.
Chloroform, methanol and ethanol extract exhibited significant antimicrobial activity and highlighted the biological monitoring of activity from the. In addition the fiber of citrus fruit also contains bioactive compounds such as polyphenols, the most important being vitamin c,and they certainly prevent and cure vitamin c deficiencythe cause of. We investigated the potency of citrus aurantifolia lime fruit, against pathogens, in the different forms in which this fruit plant is used locally juice of the fruit, burnt rind of the fruit. The phytochemical composition and comparative evaluation of antimicrobial activities of the crude juice extract and biosynthesized silver nanoparticle snps from citrus aurantifolia juice was. Swingle has been shown to have antimicrobial, antioxidants and antiinflammatory effect.
The plant is used in traditional medicine as an antiseptic, antiviral, antifungal, anthelmintic, astringent, diuretic, mosquito bite repellent. Bryophyllum pinnatum, citrus aurantifolia, synergy, antimicrobial, phytochemical constituents. Nutritional value and pharmacological importance of citrus. We investigated the potency of citrus aurantifolia lime fruit, against pathogens, in the different forms in which this fruit plant is used locally. Ortuno aa, baidez p,gomez mc, arcas i, porras ag and del rio ja. This work reports the antimicrobial effect of garlic allium sativum linn. Antibacterial activity of citrus limonon acnevulgaris. Feb 01, 2016 the hot and cold aqueous and ethanol extracts of the citrus sinensis were used for the antimicrobial screening using the agar well diffusion method. Introduction according to the world health organization, a medicinal plant is any plant in. The key lime is usually picked while it is still green, but it becomes.
Antibacterial activity of lime citrus aurantifolia essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder. Evaluation of the antimicrobial properties of different parts. Antibacterial activity of citrus aurantifolia leaves extracts. Ethnomedical importance of citrus aurantifolia christm. Rafi khan pathan1, papi reddi gali1, parveen pathan2.
Evaluation of the antimicrobial properties of different. Antibacterial activity of grapefruit citrus paradisi peel. Extracts of citrus limon were evaluated for their antioxidant and antimicrobial the highest antibacterial activity was obtained against enterococcus faecalis and bacillus subtilis most susceptible bacteria. Grapefruit, citrus limon lemon, citrus reticulata tangerine, citrus grandis shaddock, citrus aurantium sour orange, citrus medica citron, and citrus aurantifolia lime are some important fruit of genus citrus singh u et al. Antimicrobial screening antimicrobial assay of fruit and.